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C.I.P. - Chemistry of Iris Rhizomes |
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Student Name
________________________ |
PURPOSE: What chemicals are stored in Iris rhizomes?
PROCEDURE:
Cut off small pieces of root, leaf, and rhizome for each test.
STARCH:
1. Add Lugol’s (potassium iodide) solution to each piece of iris. A
black-purple color indicates the presence of starch.
GLUCOSE:
1. Mash a piece of iris with a mortar and pestle or with a stirring
rod in a plastic test tube. Add a few drops of water to moisten.
2. Place a glucose test strip (Diastix) on the moist surface. Exactly 30
seconds later compare the color of the strip to the manufacturer’s color
chart. Record the percentage of glucose for each iris part.
PROTEIN:
1. Slice off a pea sized piece of rhizome.
2. Add the rhizome to 5 ml. of Biuret solution in a test tube. Wait 15-20
minutes. A purple color indicates protein is present.
DATA:
| Leaf | Rhizome | Root | |
| Starch | |||
| Glucose | |||
| Protein |
CONCLUSION: